Instrumental operating procedures are not given because they will vary depending on instrument design. The IR technique is discussed here primarily for application in identification of organic compounds and will focus on the mid-infrared region. It can be used to quantitate analytes in complex mixtures and is used extensively in detection of industrial pollutants in the environment. IR spectroscopy is also used in quantitative techniques because of its sensitivity and selectivity. The IR spectrum of an organic compound is a unique physical property and can be used to identify unknowns by interpretation of characteristic absorbances and comparison with spectral libraries. The infrared region of the spectrum includes electromagnetic radiation that can alter the vibrational and rotational states of covalent bonds in organic molecules. Infrared (IR) spectroscopy is an absorption method widely used in both qualitative and quantitative analyses.
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